USDA/ARS/MWA - Soybean/Maize Germplasm, Pathology & Genetics Research Unit
&University of Illinois at Urbana/Champaign - Department of Crop Sciences
S-123 Turner Hall
1102 South Goodwin Avenue
Urbana, IL 61801-4730
|(217) 333-6631 [phone]
(217) 333-6064 [fax]
9,151 seed samples have been supplied in response to 458 requests for 2018 (until the shut-down in late December). These include 231 requests received from 22 foreign countries. Interest in reverse genetics tools, such as the UniformMu sequence indexed stocks, remains high and steady. Presently, requests for UniformMu stocks represent approximately 60% of our total requests. Other popular stock requests include the NAM RILs and other mapping populations, Ac/Ds sequence indexed stocks, Hi II lines, haploid-inducing lines, male sterile cytoplasms, Fast-flowering mini-maize, kernel starch quality traits, plant architecture traits, and Maize Inflorescence Project EMS lines.
Approximately Approximately 4.0 acres of nursery were grown this summer at the Crop Sciences Research & Education Center located at the University of Illinois. Warm, dry spring weather allowed us to plant both of our crossing nurseries in a timely manner. Excellent weather during the remainder of the growing season allowed for good germination in our crossing nurseries, as well as a normal pollination season; no supplemental irrigation was required, although we did irrigate our second crossing nursery to deter birds from pulling up seedlings through loose soil. There were sufficient stands for a good increase in almost all instances.
Special plantings were made of several categories of stocks:
1. Plantings were made of donated stocks from the collections of Alice Barkan (22 photosynthetic mutant allleles), Norman Best (ali1 alleles, David Braun (sut1-m4 Hugo Dooner (apt1, bz1, and nut1 alleles), Andrea Gallavotti (ad1, rel2, rte1, rte2, spi1, and ts1 alleles), Sarah Hake (gif1 alleles), Gerry Rohde (atg12 alleles), and others. We expect to receive additional accessions of stocks from maize geneticists within the upcoming year.
2. We are continuing to characterize enhancers of yellow endosperm color from PI accessions of orange endosperm tropical flints and are continuing collaborations to identify the specific gene products associated with previously uncharacterized (or incompletely characterized) white endosperm/albino seedling loci. We continue collaborations to characterize a colored pericarp crown variant from a GEM line that is unlinked to p1. Through tests of allelism, we identified new alleles of shrunken6 and albescent1. Additional tests of allelism of stocks from our Phenotype Only collection are in progress.
3. Due to lack of personnel, we continue to provide only bare-bones curation of the A-A translocation stocks and other chromosomal aberration stocks that were previously maintained by Janet Day Jackson. In most instances, we have stopped propagating them, but we will continue to provide seeds on request until they lose viability.
4. Stocks produced from the NSF project "Regulation of maize inflorescence architecture" (see: http://www.maizegdb.org/MIP/) were grown again this summer. Approximately 250 families of M2 materials that were produced between 2006 and 2013 were grown to increase seed supplies and recover previously observed mutations; this also included previously phenotyped families that had limited seed supplies. In addition, 1,070 families of 2016 and 2017 EMS seed increase materials were grown for adult plant observation and 191 families were screened in sand benches for seedling traits; the materials observed include mutated A619, B73 and Mo17 inbred lines, Mo17xA632 and B73xMo17 hybrid, and various other inbred lines.
5. Critical plantings of a limited number of stocks were made in our greenhouse facilities.
We currently have 14,033 UniformMu sequence indexed stocks, produced by the "Construction of comprehensive sequence indexed transposon resources for maize" project (see: http://www.maizegdb.org/documentation/uniformmu). We have also recently received 1,315 maize-teosinte BC1S4 population stocks from John Doebly (described in: Guo, L et al. 2018. Curr Biol 28: 3005-3015). An additional 2,215 lines of tdsg stocks from the "Ac/Ds reverse genetics resource" project (see: http://acdsinsertions.org) were added to our previous collection.
Our IT Specialist has continued to make updates and improvements to our curation tools, which are used to maintain data for our collection. These tools input our public stock data directly into MaizeGDB and our local database to give maize scientists access to up-to-date information about our collection. The current tools are basically in "maintenance mode" while work continues on a new implementation of the curation tools. Work is progressing nicely on a procedure to migrate our data from MySQL to PostgreSQL, and migration of our tools will follow soon after. Once that's done, work can commence on unified tools. Maintenance continues on our web site (http://maizecoop.cropsci.uiuc.edu/).
Agric Sci Res Tech (Plants)
Information Tech Specialist